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Does Abnormal Urine Fish Test Always Indicate Bladder Cancer

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What Does It Mean To Have Abnormal Cells In Urine

Urinary Markers in Bladder Cancer

Cells that appear abnormal in your urine may also indicate inflammation in the urinary tract or cancer of the bladder, kidney, ureter, or urethra. However, an abnormal urine cytology result cant diagnose these diseases. Additional tests are usually necessary to confirm the diagnosis.

Consequently, what is urine cytology FISH test?

Vysis® UroVysion® is a molecular cytology test that detects aneuploidy of chromosomes 3, 7, and 17 and deletion of the 9p21 locus via fluorescence in situ hybridization in urine specimens. Thus, the Vysis UroVysion test appears useful for diagnosis of bladder cancer in patients with hematuria.

What is the fish test?

Fluorescence in situ hybridization is a test that maps the genetic material in a persons cells. This test can be used to visualize specific genes or portions of genes. FISH testing is done on breast cancer tissue removed during biopsy to see if the cells have extra copies of the HER2 gene.

Can bladder cancer be detected with a urine test?

Urinalysis: One way to test for bladder cancer is to check for blood in the urine . Urinalysis can help find some bladder cancers early, but it has not been shown to be useful as a routine screening test. Urine cytology: In this test, the doctor uses a microscope to look for cancer cells in urine.

Does My Fish Have Swim Bladder Disease

Applicability of the FISH test for bladder cancer Abstract Fluorescence in situ hybridization analysis is an FDA-approved, urine-based marker that assists in diagnosis and surveillance of invasive urothelial cancer. This article provides an overview and case study demonstrating the clinical use of this analysis.

How Bladder Cancer Is Diagnosed

There are many tests used for diagnosing bladder cancer. Not all tests described here will be used for every person. Your doctor may consider these factors when choosing a diagnostic test:

  • The type of cancer suspected

  • Your signs and symptoms

  • Your age and general health

  • The results of earlier medical tests

The earlier bladder cancer is found, the better the chance for successful treatment and cure. However, there is not yet a test accurate enough to screen the general population for bladder cancer, so most people are diagnosed with bladder cancer once they have symptoms. As a result, some people have more advanced disease when the cancer is found. Still, most people are usually diagnosed with noninvasive bladder cancer .

The following tests may be used to diagnose and learn more about bladder cancer:

The following imaging tests may be used to find out if the bladder cancer has spread and to help with staging. Imaging tests show pictures of the inside of the body.

After diagnostic tests are done, your doctor will review the results with you. If the diagnosis is cancer, these results also help the doctor describe the cancer. This is called staging and grading.

The next section in this guide is Stages and Grades. It explains the systems doctors use to describe the extent of the disease and the way cancer cells look under a microscope. Use the menu to choose a different section to read in this guide.

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What The Letters Mean

The H in FISH refers to hybridization. In molecular hybridization, a labeled DNA or RNA sequence is used as a probevisualize a red Lego brick, if you will. The probe is used to find a counterpart Lego brick, or DNA sequence, in a biological sample.

DNA in your specimen is like piles of Lego bricks, and most bricks in these piles won’t match our red probe. And all of your bricks are neatly organized into 23 pairs of brick pileseach pile is one of your paired homologous chromosomes, more or less. Unlike Lego bricks, our red Lego probe is like a strong magnet and finds its match without having to sort through the piles.

The F refers to fluorescence. Our red probe might get lost in the piles of bricks, so it is labeled with colored fluorescent dye so that it will glow. When it finds its match amongst the 23 paired piles, a fluorescent tag reveals its location. So, you now can see how researchers and clinicians might use FISH to help identify where a particular gene is located for a given individual.

The I and the S stand for in situ. This refers to the fact that our red Lego brick is looking for its match within the sample you gave.

What Are The Limitations Of Urinalysis

  • A normal urinalysis does not guarantee that there an absence of cancer of the urinary system. Certain individuals will not show elevate substance or release abnormal cells in urine early enough to detected in disease process.
  • Certain individuals will release proteins into the urine at irregular intervals, such that they are lost in the sample collection.
  • If the urine is very dilute, small quantities of tumor marker proteins may be undetectable.
  • The tumor marker assays may occasionally yield false positive or false negative results so they cannot be solely rely on. Thus there is always the need for the tests to be confirm by other detection techniques.

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Can Bladder Cancer Be Detected With A Urine Test

Urinalysis can help find some bladder cancers early, but it has not been shown to be useful as a routine screening test. Urine cytology: In this test, a microscope is used to look for cancer cells in urine. Urine cytology does find some cancers, but its not reliable enough to make a good screening test.

How A Fish Test Works

In FISH testing, pieces of single-strand DNA are sent to find corresponding stretches of DNA from tumor cells sampled from the body. The probes, marked with a fluorescent dye, attach to those corresponding cellsthat process is called hybridization. Once the probes attach to the chromosomes in the tumor cells, they glow when hit with fluorescent light, clearly showing where the probes match to the cancer cells, and where any chromosomal differences are.

Each of the three types of probes have separate uses:

  • Locus-specific probes link to a certain spot on a chromosome. They may help pinpoint where a specific gene resides and the number of copies of that gene.
  • Alphoid probes or centromeric repeat probes help determine the number of chromosomes. They may be combined with locus-specific probes to see whether any genetic material is missing from a chromosome.
  • Whole chromosome probes use a combination of several probes with different color dyes to give a chromosome its own fluorescent signature. These may highlight abnormalities, such as translocation.

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Urine Culture Testing To Check For Utis

Urine culture testing can be used to check for urinary tract infections.1 The symptoms of bladder cancers and urinary tract infections can be quite similar, so it is important for healthcare providers check for both infection and cancer if either could be the cause. To carry out this test, the urine sample is left in a dish in the laboratory for several days, which allows any bacteria that may be contained in the urine to grow.

Nuclear Matrix Protein 22 Assays

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Another immunochromographic assay for BCa detection monitors urinary nuclear matrix protein 22 . NMP22 is a nuclear mitotic apparatus protein that is responsible for the distribution of chromatin to daughter cells during mitosis . As with the BTA tests, the NMP22 tests also come as a quantitative ELISA or a quantitative point-of-care test known as BladderChek . The POC test is FDA-approved for initial diagnosis, and both are approved for disease surveillance. The advantages of the POC test include low cost, ease of use, and interpretation without the need for a trained pathologist.

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What Is The Best Hospital For Bladder Cancer

Test Usage. The UroVysion Bladder Cancer Kit is designed to detect aneuploidy for chromosomes 3, 7, 17, and loss of the 9p21 locus via fluorescence in situ hybridization in urine specimens from persons with hematuria suspected of having bladder cancer. Results from the UroVysion Kit are intended for use, in conjunction with

Can Fish Detect Cll

FISH can identify chromosomal abnormalities in leukemias, including in chronic lymphocytic leukemia . 5 For chronic lymphocytic leukemia/small lymphocytic lymphoma, FISH allows patients to find out their prognostic category: good, intermediate, or poor. In acute lymphoblastic leukemia , genetics of the leukemic cells can tell you about the risk level of cancer and help to guide therapeutic decisions.

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The Contemporary Role And Impact Of Urine

Igor Duquesne1, Lars Weisbach2, Atiqullah Aziz3, Luis A. Kluth4, Evanguelos Xylinas1 on behalf of the Young Academic Urologist Urothelial Carcinoma Group of the European Association of Urology

1Department of Urology, Cochin Hospital, APHP, Paris Descartes University , Germany

Contributions: Conception and design: E Xylinas, I Duquesne Administrative support: E Xylinas Provision of study materials or patients: E Xylinas, I Duquesne Collection and assembly of data: E Xylinas, I Duquesne Data analysis and interpretation: All authors Manuscript writing: All authors Final approval of manuscript: All authors.

Correspondence to:

Keywords: Urothelial carcinoma bladder cancer screening detection surveillance urine biomarker outcomes

Submitted Oct 30, 2017. Accepted for publication Nov 21, 2017.

doi: 10.21037/tau.2017.11.29

Fish And Specific Blood Cancers

FISH and other in situ hybridization procedures are used to diagnose a variety of chromosomal abnormalitieschanges in the genetic material, changes in chromosomes, including the following:

  • Deletion: part of a chromosome is gone
  • Translocation: part of one chromosome breaks off and sticks onto another chromosome
  • Inversion: part of a chromosome breaks off and reinserts back in, but in reverse order
  • Duplication: part of a chromosome is present in too many copies within the cell

Each type of cancer may have its own set of chromosomal changes and relevant probes. FISH not only helps identify the initial genetic changes in a disease process like cancer, but it can also be used to monitor response to therapy and disease remission.

The genetic changes detected by FISH sometimes offer additional information about how an individuals cancer is likely to behave, based on whats been observed in the past in people with the same kind of cancer and similar genetic changes. Sometimes FISH is used after the diagnosis has already been made, to glean additional information that might help predict a patient’s outcome or best treatment.

FISH panels are also available for lymphoma, multiple myeloma, plasma cell proliferative disorders, and myelodysplastic syndrome. In the case of mantle cell lymphoma, for example, there is a translocation that FISH can detect called GH/CCND1 t that is frequently associated with this lymphoma.

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Benefits Of Urine Testing

Urine testing has several advantages, including:

  • Typically non-invasive and painless
  • Ease of sampling, coupled with being quick and convenient compared with other tests and procedures
  • Results can be obtained quickly
  • Costs are often lower than those associated with other types of diagnostic tests and procedures
  • Provides relevant, reliable information regarding patient health status

Transurethral Resection Of A Bladder Tumour

If abnormalities are found in your bladder during a cystoscopy, you should be offered an operation known as TURBT. This is so any abnormal areas of tissue can be removed and tested for cancer .

TURBT is carried out under general anaesthesia.

Sometimes, a sample of the muscle wall of your bladder is also taken to check whether the cancer has spread. This may be a separate operation within 6 weeks of the first biopsy.

You should also be offered a dose of chemotherapy after the operation. This may help to prevent the bladder cancer returning, if the removed cells are found to be cancerous.

See treating bladder cancer for more information about the TURBT procedure.

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Medical History And Physical Exam

Your doctor will want to get your medical history to learn more about your symptoms. The doctor might also ask about possible risk factors and your family history.

A physical exam can provide information about possible signs of bladder cancer and other health problems. The doctor might do a digital rectal exam , during which a gloved, lubricated finger is put into your rectum. If you are a woman, the doctor might do a pelvic exam as well. During these exams, the doctor can sometimes feel a bladder tumor, determine its size, and feel if and how far it has spread.

If the doctor finds things that aren’t normal, you may to have lab tests done and you might be referred to a urologist for further tests and treatment.

Urine Biomarker Tests For Bladder Cancer

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Early detection of bladdercancer can improve the chance of successful treatment. Therefore, doctors try to find urine biomarker tests, which will help to identify bladder cancer or recurrence of bladder cancer as early as possible.

Your doctor may recommend the use of urine biomarker tests, if you are considered a high-risk patient or bladder cancer is suspected from signs and symptoms, other laboratory tests or physical examination. In case of a urine biomarker test that gives a positive result, further examinations are indicated to rule out or confirm bladder cancer.

Some doctors find these urine tests useful in looking for bladder cancers, but they may not help in all cases. Most doctors feel that cystoscopy is still the best way to find bladder cancer. Some of these tests are more helpful when looking for a possible recurrence of bladder cancer in someone who has already had it, rather than finding it in the first place.

In the following section, different test systems your doctor may use are introduced. You may use this information when consulting your doctor, and when considering why an additional test may or may not be useful for you.

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How Do I Prepare For Urine Collection For Cytology Tests

  • Certain foods such as sugar beets, blackberries, or rhubarb can color urine and made it appear like it contains blood so avoid eating any of them prior to the test.
  • Tell your doctor if you are on medication such as vitamin B, Pyridium and Dilantin an . These drugs will also color the urine.
  • If your monthly cycle falls within the date you are ask to give a urine sample, let your doctor know, so you can be re-schedule to give your urine sample at a later date.
  • Drink enough water prior to giving a urine sample so you can have enough urine volume give midstream urine.

Understanding Invasiveness And Grade

Two important pieces of information to know about this specific cancer are its invasiveness and grade.

Invasiveness describes how deep the cancer is in the bladder wall, which is crucial to determining treatment. If the cancer is in the inner cell layers, its noninvasive or superficial. If its grown into deeper bladder layers or spread to other organs or lymph nodes, its invasive.

Grade describes how bladder cancer cells look under a microscope. Low-grade cancers, also called well-differentiated cancers, resemble regular bladder cells. High-grade, poorly differentiated or undifferentiated cancers dont look as much like normal bladder cells, and theyre more likely to become invasive or spread to other body parts.

Invasive and high-grade cancers may be harder to treat than noninvasive and low-grade ones. They may also require a different kind of treatment.

Its also important to know the stage of bladder cancer, or how much the cancer has spread. If the cancer has spread to other parts of the body, its called metastatic.

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How Is Urine Collected For Cancer Cytology Tests

  • Urine is usually collected at your doctors office, clinic or a lab, but if it is 24-hour urine collection, you will be shown the procedure you will need for home collection.
  • In general, it is advisable to collect first-morning urine.
  • You may be provided with sterile, clean catch kit by your doctor containing a cleansing solution, sterile wipes, and collection container.
  • You wash your hands first, open the kit, clean your genital area and then begin urinating.
  • After the urine has flowed about half way, you collect midstream into the container, as you continue with the urine flow in the toilet until completion.
  • Careful tighten the lid on the urine container and return urine sample to your doctor.
  • Your urine sample will be sent to a lab within an hour for examination.

A urine sample can also be obtained during cystoscopy procedure .

Urine Fish For Detection Of Urothelial Cancer

Fluorescence in situ hybridisation can identify urothelial carcinoma cells naturally exfoliated into the urine and its clinical use constitutes both a surveillance tool and an accurate diagnostic assay

Lorenza Pecciarini PhDEmail: The UroVysion® testUncertain urine FISH resultsUrine FISH sensitivityFISH test for upper tract urothelial carcinomaOur Institution experienceReferences

  • Phillips JL, Richardson IC. Aneuploidy in bladder cancers: the utility of fluorescent in situ hybridization in clinical practice. BJU Int 2006 98:337.
  • Krause FS et al. Clinical decisions for treatment of different staged bladder cancer based on multitarget fluorescence in situ hybridization assays? World J Urol 2006 24:41822.
  • Tsuchiya KD. Fluorescence in situ hybridization. Clin Lab Med 2011 31:52542.
  • Inoue T et al. Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization: Clinical implication for the detection of bladder cancer. Urol Res 2000 28:5761.
  • Sokolova IA et al. The development of a multitarget, multicolor fluorescence in situ hybridization assay for the detection of urothelial carcinoma in urine. J Mol Diagn 2000 2:11623.
  • Bubendorf L et al. Multiprobe FISH for enhanced detection of bladder cancer in voided urine specimens and bladder washings. Am J Clin Pathol 2001 116:7986.
  • Mbeutcha A et al. Current status of urinary biomarkers for detection and surveillance of bladder cancer. Urol Clin North Am 2016 43:4762.
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    Cxbladder Is A Genomic Urine Test For Bladder Cancer That Improves Overall Detection Accuracy

    Cxbladder is a non-invasive and easy-to-use genomic urine test that quickly and accurately detects or rules out bladder cancer. The test combines clinical risk factor markers with genetic information, measuring five biomarker genes to detect the presence or absence of bladder cancer.

    When should you use Cxbladder?

    • When you’ve seen blood in your urine
    • If tests reveal you have blood in your urine
    • When you have had bladder cancer and are being monitored for recurrence

    Cxbladder provides greater certainty, resolving diagnostic ambiguity and improving overall detection accuracy. The use of Cxbladder can also reduce the need for further invasive procedures and testing.

    Bladder cancer has a high risk of recurrence so patients who have been treated have unique monitoring needs to protect against the threat of the disease returning. Besides monitoring for signs and symptoms of bladder cancer, a cystoscopy to examine the inside of the bladder and urethra is recommended every 312 months for several years after completing bladder cancer treatment, depending on the risk of recurrence. For many patients, the frequency of cystoscopy required can be reduced with the use of Cxbladder, a non-invasive surveillance alternative.

    Cxbladders proven accuracy makes it a reliable choice. With performance proven in over 10 peer-reviewed studies, Cxbladder is trusted by over 1,800 urologists in over 40,000 patients. The test is covered by Medicare and comes with the option of in-home sampling.

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