Immunofluorescence Staining Of Bladder Sections
Sections of paraffin-embedded tissue were deparaffinized and rehydrated and pretreated with 0.3% Triton X-100/PBS at room temperature. Thereafter, sections were blocked for 30 mins with FX Signal Enhancer sections were blocked for an additional 60 mins with the sera from the species in which the secondary antibodies were raised. Incubation with primary antibodies was carried out overnight at 4 °C. Primary antibodies used were goat anti-claudin-14 and mouse anti-occludin . Sections were then incubated with secondary Alexa Fluor-conjugated antibodies for 60 mins at room temperature and mounted in ProLong Gold Antifade mounting medium including DAPI . Tissue was analyzed with a Leica SP5 confocal microscope and quantified with ImageJ software.
Get A Daily Dose Of Sunshine
While its true that too much time unprotected in the sun can be harmful, fifteen to twenty minutes of sunshine each day during the hours when the sun is strongest will help ensure you get Vitamin D through its most basic resource: the sun. Try eating your lunch out in the sun or going for a quick walk in the afternoon to get in some sun-soaking time.
Urine Analysis In Patients
When checking indicators of infection, while evaluating urine analysis, there are several factors to consider. The presence of bacteria per high power field is the most common indicator of bacterial infection. Although some amounts of bacteria in urine may be present for any patient with symptoms, according to the definition, 5+ is considered as the standard for bacteriuria. Sometimes 2+ is also considered positive in some selective populations which are hospitalized and catheterized patients The bacterial invasion determined by general urine examination and culturing revealed that out of 75 urine specimens collected from patients complaining of signs and symptoms of UTIs, Fifty two samples were positive for bacterial infection whereas 30.66% negative. From positive cultures different bacterial type isolated, most cases were due to Escherichia coli .
You May Like: Prostate Cancer Spread To Bladder Neck
Vitamins That Irritate The Bladder
While some vitamins can help with bladder health, its important to know that others can worsen your symptoms if you have bladder troubles.Nonetheless, some vitamins you may wish to avoid are:
- Vitamin C many patients with interstitial cystitis report that taking a Vitamin C supplement causes bladder flares and trigger a need to go in women. However, Vitamin C found naturally in food doesnt appear to cause issues
- Iron supplements excess iron can cause constipation, which puts pressure on the bladder, causing leaks and discomfort1
- -cryptoxanthin this is a vitamin found in food such as tangerines, red peppers and pumpkin.2 In a 2016 study, it was found to worsen voiding symptoms in individuals with lower urinary tract symptoms3
- Any vitamin buffered with aspartame may increase urinary frequency and urgency4
iD would recommend you speak to a doctor before making changes to your diet or taking supplements.
The Role Of Vitamin D In Urological Health
Vitamin D is a well-known factor in bone health however, its role in the body extends far beyond. Recent studies point to links between vitamin D and urological health.
Our bodies make vitamin D through a process that begins when sunlight converts a chemical in our skin into pre-vitamin D3 . We also get vitamin D from food, including fortified milk products, fortified cereal and fatty fish . In Canada, vitamin D is also added to margarine and egg products.
Below are some of the urological impacts of vitamin D.
Overactive bladder
Studies have found that low vitamin D levels are linked to overactive bladder.1 Overactive bladder is characterized by frequent urination, incontinence, nocturia and sudden, intense urges to urinate.
Urinary tract infection
Vitamin D deficiency is associated with increased risk of urinary tract infections in both adults and children.2
Bladder cancer
A recent research review concluded that low vitamin D is associated with increased risk of bladder cancer.3
Kidney stones
Many studies have found no link between vitamin D supplementation and the development of kidney stones. Other studies conclude that certain patients are more sensitive to vitamin D and may develop kidney stones in response to vitamin D supplements. Still other studies have found that patients with kidney stones are more likely to have low vitamin-D blood levels.4 Confused?
Benign prostatic hyperplasia
Sexual dysfunction
Male fertility
Recommended Reading: Difference Between Bladder And Urinary Tract Infection
In Vivo Mouse Model Of Uti
Female wild-type C57BL/6 mice were obtained from Janvier Laboratories following the standard procedures. Mice were fed with normal diet. Similarly for vitamin D depleted C57BL/6 mice, 3-week-old animals were supplied with vitamin D-deficient diet TD.89123 for 7 weeks and were housed behind a UV protection film directly after weaning, whereas control mice were fed with normal diet TD.110133 supplemented with 1.5 IU/g of cholecalciferol . Mice were anesthetized using isoflurane and transurethrally infected with 0.5×108 colony-forming units of E. coli CFT073 in 50 l of PBS. After 24 h infection, mice were sacrificed and their bladders were aseptically removed and fixed with 4% paraformaldehyde and processed for immunohistochemistry staining.
My Take On Vitamin D In Men To Promote Longevity
First of all, vitamin D is not a vitamin, its a hormone.
Vitamins are vital nutrients that come from food or other external methods like dietary supplements not made by your body.
Hormones are chemicals made from steroids or amino acids produced by the body that attaches to receptors and causes a physiological response. For example, when testosterone attaches to receptors on specific cells in teenage boys after puberty, it causes facial hair growth and a deeper voice.
Moreover, vitamin D is a steroid hormone with vitamin D receptors in virtually every cell in the human body with calcemic effect and a non-calcemic effect.
Vitamin D has two primary jobs One it increases absorption of calcium in the body primarily to maintain bone health and, two, has its numerous actions on the body independent of calcium metabolism , i.e., boost immunity, anti-inflammatory, maybe anti-cancer, etc.
Vitamin D insufficiency was defined in most studies as below 30 ng/mL, and deficiency ranges from < 20 ng/mL to 10 ng/mL.
for vitamin D is nmol/L. To convert mg/ml to nmol/L, multiply the ng/ml value by 2.5)
The most recent study associating vitamin D with LUTS is just one of a plethora of research suggesting its protective qualities.
In a 9-year follow-up study looking at over 13,000 adults 20 years or older, there was an increased rate of death in those with low Vitamin D levels .
The sweet spot, that is, where most participants died the least, was with D levels around 40 to 45ng/ml.
Don’t Miss: Prostate And Bladder Cancer Together
New Vitamin D Deficiency Danger
Vitamin D is an essential vitamin necessary for bone and muscle strength. The body produces vitamin D in response to exposure to sunlight, but the vitamin is also commonly found in fortified dairy products and supplements.
Researchers say vitamin D deficiency is already associated with an increased risk of osteoporosis, and recent studies have also linked osteoporosis to pelvic floor disorders.
This study looked at the link between vitamin D levels and pelvic floor disorders reported by 1,881 nonpregnant women over age 20 who participated in the 2005-2006 National Health and Nutrition Examination Survey.
The results showed 82% of women had vitamin D levels that were considered deficient.
One or more pelvic floor disorders were reported by 23% of the women, and average vitamin D levels were significantly lower among those with at least one pelvic floor disorder or incontinence.
Overall, increased vitamin D levels were associated with a lower risk of pelvic floor disorders.
In older women, the risk of urinary incontinence was also 45% lower among women with normal vitamin D levels.
Higher vitamin D levels were also associated with a slightly lower risk of fecal incontinence, but the relationship was not considered significant, which researchers say may be due to the low number of fecal incontinence cases reported.
Show Sources
Can Too Much Vitamin D Cause Frequent Urination
Vitamin D is a fat-soluble vitamin that helps in increased absorption and metabolism of minerals mainly Calcium, Magnesium, Phosphate and many such important biological functions. It is required for cognitive body functions, increasing immunity, maintenance of blood sugar level and mood swing. It has also seen to affect the cardiovascular health. Some studies have shown that the vitamin helps in cancer prevention and some of the new studies suggest that vitamin D deficiency may also lead to fecal and urinary incontinence or frequent urination.
Human body produces two types of Vitamin D, namely, Cholecalciferol and Ergocalciferol.
Cholecalciferol is Vitamin D3 which is produced by human skin. On exposure to UV light, Cholecalciferol is converted into Calcifediol in the liver and later into Calcitriol in the kidneys. It can also be consumed by dietary supplements and food items like fish, cheese and eggs. It helps in the increased absorption of calcium at the level of intestine. This hormone is mainly used to treat conditions like Vitamin D deficiency, X- linked hypophosphatemic rickets and hypoparathyroidism to prevent the lowering of blood calcium levels.
Ergocalciferol, also known as Vitamin D2, is mainly used as a Vitamin D supplements however, Cholecalciferol is naturally produced by human body.
Vitamin D may also lead to complications. Vitamin D is a fat-soluble vitamin, so excess of the vitamin might get stored in the body.
Recommended Reading: Can Bladder Cancer Be Seen On Ultrasound
Does Vitamin D Treat Bph
Vitamin D is not a proven treatment for BPHyet.
A study in the Canadian Journal of Urology discovered that, the impact of vitamin D on prostate volume and BPH has shown promising results, thus proposing further studies on vitamin D and BPH be conducted.
Although conclusive results are still needed, vitamin D shows some significant benefits that can reduce symptoms and potential causes of BPH
Vitamin B12 For An Overactive Bladder
Also known as cobalamin, Vitamin B12 is a water-soluble vitamin which can help to improve central nervous system functioning, relieve bladder pressure and alleviate mild urine leakage. The recommended daily dosage for vitamin B 12 is 2.4 mcg for teenagers and adults. Some foods which contain high amount of vitamin B-12 are milk, rainbow trout, plain yogurt, top sirloin beef, beef liver, salmon, white tuna, eggs, breakfast cereals, and roasted chicken. Consuming foods rich in vitamin B12 helps in dealing with an overactive bladder.
You May Like: Why Can I Not Control My Bladder
Vitamin D Plays A Role In Bladder Health
Weve been hearing a lot in the press about vitamin D lately. You probably know it plays an important role in bone health because it facilitates calcium absorption. Vitamin D also impacts heart health, immune health, mood, normal healthy blood sugar. And new research also supports that Vitamin D plays a role in bladder health.
When we take in vitamin D from food, supplements or sun, it is inert and it must undergo two different reactions in our bodies in order to become active. During this process a metabolic product known as calcitrol is formed calcitrol is believed to target more than 2000 genes, or almost a tenth of all human genes. Maybe now you can see why vitamin D is so important to our health!
Vitamin D is actually not really a vitamin: its a group of prohormones. Vitamin D2 and vitamin D3 are the two main forms. Because vitamin D is fat soluble, meaning it will only be absorbed and processed in the presence of fat, it can be stored in our fat cells, which means that even though we must eat it or get it from sunshine, our bodies are able to store excesses to draw upon them in time of need.
Vitamin D Levels Impact on Pelvic Floor Health and Bladder Control
The pelvic floor muscles are a group of muscles that span the pelvis in our body. They help to keep these muscles in the correct position. With age, or due to trauma or childbirth, the pelvic floor muscles can become weak and contribute to the sagging of organs or even to occasional leakage of urine.
Inclusion And Exclusion Criteria
Inclusion criteria were permanent residency in nursing homes for the elderly, regardless of gender and duration of residency, nursing home resident during the study, approved participation, absence of indwelling urinary catheter, ability to leave a voided urine sample and in case of dementia inclusion only if cooperative when collecting urine and blood samples.
Exclusion criteria were urostomy, terminal illness, intermittent catheterisation, ongoing antibiotic treatment and discontinued study participation.
Recommended Reading: How To Take Care Of A Bladder Infection At Home
Vitamin D For An Overactive Bladder
Vitamin D is a fat-soluble vitamin, which can help with the problem of an overactive bladder and in reducing bladder inflammation, promoting better bladder muscle contraction, strengthening bladder muscles, and decreasing the risk of pelvic floor disorders like urinary incontinence. The recommended daily intake of vitamin D is 15 mcg for adults. Some vitamin D rich foods to be eaten for alleviating an overactive bladder are fortified soy milk, canned pink salmon, sardines, fortified cereals, fortified orange juice, egg yolks, and fortified cowâs milk.
Rats Without Any Bile Had Virtually Zero Vitamin D Absorption
Bile salt deficiency and the absorption of vitamin D metabolites. In vivo study in the rat.Isr J Med Sci. 1987 Nov 23:1114-7.Maislos M1, Shany S.
We studied the effect of the absence of bile salts on the absorption of vitamin D metabolites in vivo, in the biliary duct-ligated rat. The mesenteric, lymphatic absorption of the metabolites studied was almost completely abolished in the experimental animals. These results differed significantly from those of the control rats. The 1,25-dihydroxyvitamin D3 absorption into the portal vein system was unaffected by the lack of biliary salts. The absorption of 25-hydroxyvitamin D3 was decreased and that of vitamin D3 was negligible, under the same experimental conditions. These data show that the more polar vitamin D metabolites, like 1,25-dihydroxyvitamin D3 and to some extent 25-hydroxyvitamin D3, are absorbed directly into the portal blood without the involvement of bile salts and micelle formation. Thus, the use of polar vitamin D metabolites should be considered in correcting hypovitaminosis D and osteomalacia in cases of chronic biliary salt depletion.
PMID: 2830204
Also Check: Stress Incontinence Vs Overactive Bladder
Rats Without Any Bile Had Virtually Zero Vitamin D Absorption 1987
Bile salt deficiency and the absorption of vitamin D metabolites. In vivo study in the rat.Isr J Med Sci. 1987 Nov 23:1114-7.Maislos M1, Shany S.
We studied the effect of the absence of bile salts on the absorption of vitamin D metabolites in vivo, in the biliary duct-ligated rat. The mesenteric, lymphatic absorption of the metabolites studied was almost completely abolished in the experimental animals. These results differed significantly from those of the control rats. The 1,25-dihydroxyvitamin D3 absorption into the portal vein system was unaffected by the lack of biliary salts. The absorption of 25-hydroxyvitamin D3 was decreased and that of vitamin D3 was negligible, under the same experimental conditions. These data show that the more polar vitamin D metabolites, like 1,25-dihydroxyvitamin D3 and to some extent 25-hydroxyvitamin D3, are absorbed directly into the portal blood without the involvement of bile salts and micelle formation. Thus, the use of polar vitamin D metabolites should be considered in correcting hypovitaminosis D and osteomalacia in cases of chronic biliary salt depletion.
PMID: 2830204
Vitamin D And Urinary Tract Infection: A Systematic Review And Meta
Read Also: Early Symptoms Bladder Cancer Woman
Gallbladder Removal And Vitamin D Deficiency
- Gallbladder surgery is very common: 500,000 – 750,000 in US each year roughly 3% of adults have had gallbladder surgery
Many ways to get vitamin D if gallbladder had been removed or is functioning poorlyGut friendly Vitamin D, sublingual, topical, from the sun, from UV lamps, etcPerhaps also water soluable Vitamin D, such as from Bio-Tech
Vitamin D level was 25 percent lower if lack a gall bladder March 2018
25-Hydroxyvitamin D levels and bone mineral density evaluation in patients with cholecystectomy: a case-control study.Arch Osteoporos. 2018 Mar 2 13:14. doi: 10.1007/s11657-018-0435-7.Ekiz T1, Yeen SF2, Katar MK2, Genç Ö3, Genç S3.1 Department of Physical and Rehabilitation Medicine, Elbistan State Hospital, Karaelbistan, 46300, Kahramanmara, Turkey. .2 Department of General Surgery, Elbistan State Hospital, Kahramanmara, Turkey.3 Department of Internal Medicine, Elbistan State Hospital, Kahramanmara, Turkey.
PMID: 29500745 DOI: 10.1007/s11657-018-0435-7
Irregular Heartbeat And Heart Attack
Extreme hypercalcemia can reduce or change the ability of the hearts cells to function, and people with severe hypercalcemia often experience irregularities in the heartbeat.
A person with severely high levels of calcium or phosphate in the blood may also develop calcium deposits, or plaques, in the arteries or valves of the heart.
In 2012, doctors the case of a 70-year-old woman who received calcium injections in the equivalent of 80,000 IU/day over 30 days.
She experienced hypercalcemia and complete heart blockage, which required a permanent pacemaker.
Some signs of heart complications associated with vitamin D toxicity include:
- an irregular heartbeat, which may be temporary or continual
- chest pain
- severe back or joint pain
- loss of height or length of limb
Read Also: Natural Remedies To Cure Bladder Infection
Eat Vitamin D Rich Foods
There are many foods which naturally contain higher levels of Vitamin D.
- Fatty fish such as salmon and sardines contain high levels of Vitamin D
- Grass-fed, free range beef contains higher levels of Vitamin D, especially in the liver
- Dairy products such as milk and butter from grass-fed cows contain higher levels of Vitamin D
- Eggs from free-range hens contain higher levels of Vitamin D
Quality foods such as the ones listed above naturally contain higher levels of Vitamin D, however the overly-processed foods that are on most store shelves these days often diminish the levels of Vitamin D in the foods that naturally carry higher levels of Vitamin D. For this reason its smart to purchase grass-fed and free-range meat, chicken and dairy products when possible, and to avoid fish with higher levels of mercury. Alaska-caught wild salmon is the perfect example of a quality fish.